Category:Indian films
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Category:Tamil remakes of Malayalam filmsL-triiodothyronine modulates Na(+)-K(+)-ATPase and Na(+)-K(+)-Cl- cotransporter proteins in kidneys of rats with hyperthyroidism.
Hyperthyroidism is known to increase renal Na(+)-K(+)-ATPase (NKA) and Na(+)-K(+)-Cl- cotransporter (NKCC) activities. Recent studies suggest that thyroid hormones interact with multiple targets and induce multiple metabolic responses, but the effect of thyroid hormones on renal NKCC and NKA in hyperthyroidism remains uncertain. This study was designed to investigate the renal NKCC and NKA protein expression in hyperthyroidism. Adult male Wistar rats were injected with L-triiodothyronine (1 mg/kg body weight) daily for 5 days. Glomeruli were isolated by the sequential pronase-collagenase perfusion method. Isolated glomeruli were divided into three groups: control (n = 12), L-triiodothyronine-treated (n = 12), and hypothyroid (n = 12). NKA and NKCC expression were analyzed by Western blot and immunofluorescence. The expression of Na(+)-K(+)-ATPase was increased in hyperthyroid rats compared with control rats. NKCC expression was increased in the apical membrane of tubular cells. In hyperthyroid rats, the cytosolic NKCC protein expression in the apical membrane of tubular cells was increased compared with control and hypothyroid rats. The expression of Na(+)-K(+)-ATPase in hyperthyroid rats was decreased compared with control rats. Furthermore, we investigated the effect of L-triiodothyronine on NKA and NKCC expression after a single dose. Hyperthyroid rats were treated with L-triiodothyronine (1 mg/kg body weight) and renal tissue was isolated. The results showed that L-triiodothyronine decreased NKA expression. On the other hand, L-tri be359ba680
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